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Help with Sample Sheet Preparation for Methyl-seq (S4 Illumina EM-seq) #510

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CHOWDHURY098 opened this issue Mar 19, 2025 · 1 comment
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enhancement New feature or request

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@CHOWDHURY098
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Description of feature

Issue Description:
Hello,

I am working with S4 Illumina EM-seq data for Methyl-seq analysis. My dataset consists of paired normal and tumor samples, with each patient having 4 sequencing lanes (8 reads in total per patient).

I need help with formatting the sample sheet correctly for processing. Could you provide guidelines or an example format for structuring it?

Data Details:

Platform: Illumina S4 (EM-seq)
Sample type: Paired (Normal & Tumor)
Each patient: 4 lanes, 8 reads total
File structure: FASTQ files for each lane

Any help or example sample sheet format would be greatly appreciated. Thanks!

@CHOWDHURY098 CHOWDHURY098 added the enhancement New feature or request label Mar 19, 2025
@sateeshperi
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Hi you can find that info in the usage docs here or if you look in the repo assets/samplesheet.csv is the one we use for testing

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